Compared to polyacrylamide gel, agarose has a lower resolution but wider range of separation. Lower grades of agarose can be contaminated with other polysaccharides, salts and proteins. Such impurities can alter the gelling/melting temperature of agarose solutions or affect the ability to use the recovered nucleic acid sample in a post-electrophoresis application.
Fisher Bioreagents
High-quality, high-purity, and pre-qualified reagents that meet your life science needs, all from one source.
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Fisher Bioreagents: Your Source for Life Science Products
Obtaining optimal results in your life science research requires careful selection of reagents. When your experiments require exact conditions, you can depend on the reliability of Fisher Bioreagents™ products for:
- QUALITY: Tight specifications
- CONSISTENCY: Lot-to-lot uniformity
- SELECTION: Powders, granules, concentrated stock solutions, or ready-to-use liquids
- PACKAGING: State-of-the-art containers designed for safety and utility
- CONVENIENCE: Prequalified for a variety of applications
- ECONOMY: Configurations to meet all budgets
- SCALE: From bench to batch sizes
Fisher Bioreagents meet the needs of every budget and scale. Economical powders come in a variety of package sizes, while concentrated stock solutions offer convenient one-step dilution. Realise the greatest possible time savings when you choose a Fisher Bioreagents ready-to-use solution.
Restriction Enzymes
Resources
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- 16475_fisher-scientific-bioreagents-relative-activity-optizyme-guide.pdf
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- 57 KB
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- application/pdf
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- 16475_fisher-scientific-bioreagents-reaction-buffers-optizyme-guide.pdf
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- 64 KB
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- application/pdf
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- 16475_fisher-scientific-bioreagents-recognition-sequences-optizyme-guide (1).pdf
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- 62 KB
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- 16475_fisher-scientific-bioreagents-site-specific-methylation-optizyme-guide.pdf
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- 98 KB
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Bioreagent Buffers
Buffers for Every Budget and Scale
- Economical powders come in various package sizes
- Concentrated stock solutions provide convenience with one easy-dilution step
- Ready-to-use solutions offer the greatest possible time savings
Technical Tip
CellPURE Biological Buffers are of the highest quality (purity ≥99%). They are tested for low heavy metal content and the absence of endotoxins, nucleases, and proteases. It is important to use only water of the highest quality to prepare the buffer solutions. Water that stands too long in pipes or water produced by a system needing routine maintenance increases the risk of contamination of buffer solutions. Fisher BioReagents provides several grades of high-purity water suitable for the preparation of high-quality buffer solutions.
Resources
- Filename
- 16475_Vital_Reagents.pdf
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- 2 MB
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- application/pdf
- Filename
- Fisher_Bioreagents_Buffer_Guide.pdf
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- 14 MB
- Format
- application/pdf
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Electrophoresis
Fisher Bioreagents offers a wide range of reagents for electrophoresis applications. Choosing the right reagents will allow you to achieve optimal results in your experiment. Use our quick selection guides to assist you in finding the right grades of agarose, buffers and ladders.
Fisher BioReagents offers three different grades of agarose that are functionally tested and pre-qualified for specific applications. Agarose grades for use in electrophoresis of nucleic acids include:
- Genetic Analysis Grade - Agarose that yields biologically active DNA or RNA. Testing includes enzymatic performance measurements
- Molecular Biology Grade - Suitable for analytical separation of DNA or RNA
- PCR Grade - The original agarose for analytical separation of PCR amplicons (<1kb)
| Type of Agarose | Low EEO | Low Melting | Wide Separation Range | PCR Grade |
|---|---|---|---|---|
| Recovery of DNA and RNA | X | X | X | X |
| Southern and Northern Biots | X | |||
| DNA/RNA Separation 50bp to 1kb | X | |||
| DNA/RNA Separation >1kb | X | X | X | |
| PCR Fragment Analysis | X | X | X | X |
| In-Gel Reactions (Ligation, Transformations, PCR) | ||||
| Colony Lifts | X | |||
| Available Pack Sizes | 100g to 500g | 25g | 100g to 500g | 100g |
| Agarose Grade | Molecular Biology | Molecular Biology | Genetic Analysis | PCR |
Two buffers commonly used for DNA agarose electrophoresis are Tris-acetate with EDTA (TAE; 40mM Tris-acetate, 1mM EDTA) and Tris-borate with EDTA (TBE, 89mM Tris-borate, 2mM EDTA). TAE and TBE have different properties, which makes one more suitable than the other for a specific purpose.
Fisher BioReagents provides a wide range of standards, including routine DNA ladders for quick size and quality assessment as well as Fisher Bioreagents™ exACTGene™ DNA ladders that allow for quantitative analysis.
Fisher Bioreagents™ RiboLadders™ RNA Standards
These standards can be used to assess single-stranded RNA molecules on both native and denaturing agarose gels. These unique RNA standards are lyophilized to reduce thawing-related degradation, to prolong shelf life, and to ensure consistent performance.
exACTGene and Routine DNA Ladders
Ready-to-use (pre-mixed with the loading dye), room temperature, stable DNA ladders are available for all common electrophoresis applications.
| Application | Low EEO | Numbers of Bands | Number of Loadings |
|---|---|---|---|
| exACTGene DNA ladders are ideal for qualitative analysis, quantitative estimation, and size assessment | |||
| PCR fragment analysis | 25-650bp | 14 | 100/10μL |
| PCR fragment analysis, small DNA digests | 25-1,000bp | 12 | 100/10μL |
| Quick check of PCR or enzyme digestion results | 50-2,000bp | 8 | 100/10μL |
| General purpose, small DNA fragments | 100-1,000bp | 10 | 100/10μL |
| Fast run times, small DNA fragments | 100-2,000bp | 8 | 100/10μL |
| Clone identification | 100-2,686bp | 14 | 100/10μL |
| Large size PCR or cloning | 100-2,000bp | 10 | 100/10μL |
| Small or large cloning application | 100-5,000bp | 16 | 100/10μL |
| General purpose, large digested DNA | 300-10,000bp | 13 | 100/10μL |
| General purpose, wide size range | 100/10,000bp | 19 | 100/10μL |
| General purpose, extra-large fragments | 300-24,000bp | 15 | 100/10μL |
| Routine DNA ladders are designed for qualitative analysis and size assessment | |||
| Small fragments, quick size assessment | 50-2,000bp | 11 | 200/5μL |
| Quick size assessment of broad size range | 50-10,000bp | 16 | 200/5μL |
Fisher Bioreagents
Fisher Bioreagents
Within our Thermo Fisher Scientific Laboratory Chemicals portfolio we offer all the necessary products to prepare lysis buffer solutions with the correct specifications and in a range of convenient pack sizes. We also offer specialized custom services and can provide the products in bulk quantities if required.
- Filename
- Lysis_Buffers.pdf
- Size
- 5 MB
- Format
- application/pdf
This troubleshooting guide for cloning includes a listing for problems, causes, and solutions.
- Filename
- fisher-scientific-bioreagents-troubleshooting-cloning-guide.pdf
- Size
- 67 KB
- Format
- application/pdf
This guide lists the activities of OPTIZYME restriction enzymes when their target sites are located close to the ends of PCR products.
- Filename
- fisher-scientific-bioreagents-cleavage-efficiency-pcr-fragments-guide.pdf
- Size
- 52 KB
- Format
- application/pdf
This guide lists the enzymes that may exhibit star activity, especially under reaction conditions that deviate from those recommended.
- Filename
- fisher-scientific-bioreagents-star-activity-enzymes-guide (1).pdf
- Size
- 68 KB
- Format
- application/pdf
Troubleshooting Guide for DNA Digestion: Unexpected Cleavage Pattern and Gel Shift. Table lists possible causes and recommended solutions.
- Filename
- fisher-scientific-bioreagents-troubleshooting-cleavage-pattern-guide.pdf
- Size
- 54 KB
- Format
- application/pdf
Fisherbrand, Fisher Chemical and Fisher BioReagents working together to deliver reliable and essential products that meet your most demanding electrophoresis applications.
- Filename
- 13094_FOCUS_ph_electrophoresis (1).pdf
- Size
- 114 MB
- Format
- application/pdf
This guide for digestion of agarose embedded DNA includes a listing of units of enzymes needed for DNA cleavage in 16hours.
- Filename
- fisher-scientific-bioreagents-digestion-agarose-dna-guide (1).pdf
- Size
- 46 KB
- Format
- application/pdf
Incomplete digestion or no digestion. Table includes a listing of possible causes and recommended solutions.
- Filename
- fisher-scientific-bioreagents-troubleshooting-dna-digestion-guide.pdf
- Size
- 80 KB
- Format
- application/pdf
Molecular diagnostics is the fastest-growing field of laboratory science. Fisher BioReagents products are ultrapure and tested for molecular applications.
- Filename
- fisher-scientific-bioreagents-molecular-diagnostics-guide (1).pdf
- Size
- 724 KB
- Format
- application/pdf
SurePrep Purification Kits for isolating RNA, DNA, and proteins in clinical, forensic, and medical research.
- Filename
- fisher-scientific-bioreagents-sureprep-purifications-kits-guide.pdf
- Size
- 1002 KB
- Format
- application/pdf
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