Protein Biology Methods
Genes direct the production of proteins that play a significant role in diseases such as cancer, Alzheimer’s, and diabetes. Protein biology helps us understand the output of these genes and the way they influence our health. Through protein isolation, purification, separation, and detection, protein biology provides earlier and more accurate diagnoses.
Step 1: Extraction
Isolate proteins from your mixture with the products below. This protein biology method will help you start your process and prepare you for future workflow steps. Find protein extraction essentials, lysis reagents, plus additional products and resources.
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- Pall_AcroPrep_24_Well_Filter_Plates_Application_Note.pdf
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- 2 MB
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- application/pdf
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- Pall_AcropPrep_24_Well_Filter_Plate_Family.pdf
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- 2 MB
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- Pall_Centrifugal_Devices.pdf
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- 3 MB
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- Benchtop_Instrument_Portfolio.pdf
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- 2 MB
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Step 2: Clean Up
Once extracted, the sample needs to be cleaned of the impurities that can hinder downstream sample analysis. We offer the full clean up workflow, from dialysis to desalting and concentration.
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- Thermo_Scientific_Pico_and_Fresco_Microcentrifuge_Series.pdf
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- 21 MB
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- application/pdf
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- Eppendorf_Catalogue_2022_English.pdf
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- 22 MB
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- application/pdf
Step 3: Purification
Purification is another necessary protein biology method and involves separation by protein binding, washing, and elution. The protocol used will depend on the protein you’re purifying. To get started, shop prepacked columns, products for protein purification, dialysis and desalting, and endotoxin removal. For further information, download our resources.
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- Pierce protein and peptide assay selection guide.pdf
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- 4 MB
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- application/pdf
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- Protein_Assay_Technical_Handbook.pdf
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- 13 MB
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- IBA_Protein_Production_And_Assays.pdf
- Size
- 4 MB
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- application/pdf
Step 4: Separation
Solubility, size, charge, and other protein characteristics are used to separate them. These techniques include various forms of chromatography in addition to electrophoresis and dialysis. Find the products you need for each of these methods and more by browsing gels, columns, and reagents. Learn more by downloading our relevant resources.
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- Unique_Protein_Gel_for_your_every_Need.pdf
- Size
- 7 MB
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- application/pdf
Step 5: Protein Detection
Western blotting is frequently used for protein detection. Browse related reagents, substrates, and imaging systems to help advance your protein detection protocol.
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- Bandmate_Automated_Western_Blot_Processor.pdf
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- 686 KB
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- BOOST_your_DNA_analysis.pdf
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- 544 KB
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