Cell Culture
1. Sample Preparation
Preparing samples for PCR or qPCR typically requires extraction and purification of nuclear material. Depending on the sample source, this may involve mechanical manipulation via homogenization or require the use of resins or beads to help bind the nucleic acids prior to purification. Prepare your samples with the help of DNA and RNA extraction, isolation, and purification kits, plus reagents, buffers, and more.
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- 25524_Cell_Culture_Workflow.pdf
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- 25524_Essentials_for_Life_Science.pdf
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- 25524_Thermo_Scientific_Sorvall_BIOS_Centrifuge_Series.pdf
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- 25524_gibco-cell-culture-basics-handbook.pdf
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2. Cell Expansion
Your next step, cell expansion, is necessary for cell growth and will take place in a vessel containing a culture medium. There are multiple options available based on the cell line being studied. To prepare, shop products for cell expansion including media, sera, and flasks for your research. For more information on these items, download the related resources.
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- Gibco-fbs-one-shot-brochure.pdf
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- Thermo_Scientific_Aqua-Tec_Water_Preservation_Cell.pdf
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- Thermo_Scientific_Forma_Steri-Cycle_Cleanroom_Incubators.pdf
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- Thermo_Scientific_Heracell_Cleanroom_Incubators.pdf
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- What_is_Certified_Cleanroom_Compatible_Incubators.pdf
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3. Harvesting
Collecting and isolating cells may involve centrifugation, depth filtration, and cell detachment. Before proceeding, adherent cells should be detached from the surface of the cell culture flask or dish. Choose to harvest your cells with products from our portfolio. Find cell scrapers, lifters, and reagents, as well as resources to support your workflow.
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- Thermo_Scientific_Medifuge_Centrifuge.pdf
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4. Detection
Now that your cells are harvested, determine how you’ll read your results. There are many equipment options available to make this process more accurate and efficient. Buy counters, readers, and more to detect cells and cell colonies. Learn about microplate selection and imaging with our additional resources.
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- Agilent_BioTek_Multimode_Microplate_Readers.pdf
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- 2 MB
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- Agilent_BioTek_Selection_Guide.pdf
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- 2 MB
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5. Analysis
Begin to study your cells through observation and analysis. With the right equipment, you can make this step of your workflow more effective. Choose from a variety of microscopes and microplate washers and readers for your cell culture analysis. For more information, browse our brochures and buying guides or visit our cell analysis page.
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- Agilent_BioTek_Multimode_Microplate_Readers.pdf
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6. Storage
There are many product options and techniques to choose from when storing your cells. Cryopreservation, for example, helps you keep cells safe long term for future research projects. Find vials, microplates, and media for cell storage. Make these products part of your workflow by learning more and placing an order.
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